Figure 13.

(a) Size exclusion radio-HPLC traces of [⁶⁸Ga(THP-PhNCS-trastuzumab)] (red) and [⁶⁸Ga(EDTA)]⁻ (black); and (b) In vitro uptake of [⁶⁸Ga(THP-PhNCS-trastuzumab)] and [⁶⁸Ga(THP-PhNCS-trastuzumab)] in the presence of an inhibitory concentration of trastuzumab, and ⁶⁸Ga³⁺ in HER2-positive HCC1954 cells, and [⁶⁸Ga(THP-PhNCS-trastuzumab)] in HER2-negative A375 cells, after 30 min incubation. Uptake is expressed as a percentage of added radioactivity (AR)/1 × 10⁶ cells (n = 3, error bars correspond to standard error of the mean).