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. 2017 Jan 17;18(1):157. doi: 10.3390/ijms18010157

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© 2017 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Change of claudin-2, LC3B-II, and P62 protein in TNF-α treated Caco-2 cell monolayers. (A) Western blotting analysis of caludin-2 in Caco-2 cell monolayer after TNF-α (10 ng/mL) administration or 3-MA (5 mM) treatment for 48 h. (B,C) Western blotting analysis of LC3B-II and P62 in Caco-2 cells after TNF-α (10 ng/mL) administration for 48 h. β-actin was used as an internal control. Data were shown as mean ± SD and replicated three times. ** p < 0.01 versus control group. Ctr, control group.