Table A1.

Primer sequences and probes used for real-time PCR analysis.

Gene	Oligonucleotide Sequence	Probe
GAPDH	5′-AGCCACATCGCTCAGACAC-3′/5′-GCCCAATACGACCAAATCC-3′	60
RUNX2	5′-CACCATGTCAGCAAAACTTCTT-3′/5′-TCACGTCGCTCATTTTGC-3′	41
Osteocalcin	5′-GGCGCTACCTGTATCAATGG-3′/5′-TCAGCCAACTCGTCACAGTC-3′	1
Osteonectin	5′-GTGCAGAGGAAACCGAAGAG-3′/5′-TGTTTGCAGTGGTGGTTCTG-3′	77
ALPL	5′-AGAACCCCAAGGCTTCTTC-3′/5′-CTTGGCTTTTCCTTCATGGT-3′	31
Type I collagen alpha 1	5′-CCCCTGGAAAGAATGGAGAT-3′/5′-AATCCTCGAGCACCCTGAG-3′	60
Osterix/SP7	5′-GACTGCAGAGCAGGTTCCTC-3′/5′-TAACCTGATGGGGTCATGGT-3′	43