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. 2017 Jan 14;18(1):163. doi: 10.3390/ijms18010163

Figure 2.

Figure 2

The hybridization on the microarray chip. (A) Identification of genes potentially involved in the multidrug resistance (MDR) phenotype. RNA from MCF-7 cells and MCF-7/MX cells was reverse transcribed and labeled with Cy3-dCTP (MCF-7; green) and Cy 5-dUTP (MCF-7/MX; red). Genes up-regulated in MCF-7/MX appear red and those that are down-regulated appear green. A portion of the cDNA microarray is magnified on the right to show the element representing the ATP synthetase α subunit (α ATP synthase) and COX6C genes, which are over-expressed only in the multidrug-resistant MCF-7/MX cells; (B) mRNA level of ATP synthetase α subunit (α ATP synthase) and cytochrome c oxidase subunit VIc (COX6C) in MCF-7 and MCF-7/MX cell lines; (C) Protein level of ATP synthetase α subunit (α ATP synthase) and cytochrome c oxidase subunit VIc (COX6C) in MCF-7 and MCF-7/MX cell lines.