Figure 4.

NFκB expression is affected by caffeine administration. (Box) Stimulation of the NFκB pathway is mediated by diverse signal transduction cascades. The change of protein expression of NFκB were measured in brain homogenates of normoxia (white bars), caffeine with normoxia (light grey bars), hyperoxia (black bars), and hyperoxia with caffeine (dark grey bars) by Western blot and quantitative real-time PCR with: (A) NFκB protein expression in cytosolic (cyt) and nuclear (nuc) fraction; and (B,C) NFκB mRNA expression after 24 h and 48 h of oxygen exposure. Data are shown as mean ± SEM, n = 5 per group per time point. The 100% value is: (A) (cyt) 0.035 and 0.024 ratio intensity/mm² and (nuc) 0.092 and 0.127 ratio intensity/mm²; (B) 1.017 and 1.028 C_T; and (C) 1.010 and 1.032 C_T for 24 and 48 h groups, respectively. Data were analyzed by two-way ANOVA with Bonferroni post hoc test, with * p < 0.05, ** p < 0.01, and *** p < 0.001 versus control (atmospheric air), and ^## p < 0.01 and ^### p < 0.001 versus hyperoxia (80% oxygen without caffeine).