Figure 2.

CBDL induces a pro-apoptotic response. (a) Real-time qPCR analysis of caspase 3, caspase 12, tumor necrosis factor receptor superfamily, member 1A (TNFRsf1a) and Fas-Associated protein with Death Domain (FADD); (b) Real time qPCR analysis of caspase 1; (c) Western blot of nucleotide binding, leucine-rich repeat and pyrin domains-containing protein 3 (NLRP3) on whole liver lysates. Western blot results are representative of all samples: samples of three to five mice were mixed for blotting and two different pools of the CBDL samples were blotted. Quantification of NLRP3 was normalized to total protein load. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.