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. 2017 Jan 20;18(1):214. doi: 10.3390/ijms18010214

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© 2017 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Treatment with TUDCA reduces CBDL-induced CHOP and pro-apoptotic markers. (a) Representative Western blot of CHOP on whole liver lysates. β-Tubulin was used for normalization. Quantification of CHOP blot compared to β-tubulin bands. Samples of three to five mice per group were mixed for blotting; (b) Real-time qPCR analysis of pro-apoptotic genes caspase 3 and 12, TNFRSf1a, FADD and Bcl2. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001; (c) Western blot and quantification of cleaved caspase 3 and cleaved caspase 12 on whole liver lysates. β-Tubulin was used for normalization. One to two samples per group were loaded with one sample consisting of a mixture of two mice; (d) Real-time qPCR analysis of pro-pyroptotic marker caspase 1. Data are presented as mean ± SD; (e) Western blot of NLRP3 on whole liver lysates. Quantification of NLRP3 was normalized to total protein load. Three samples per group were loaded with one sample consisting of a mixture of two mice, so a total of six mice were analyzed.

Treatment with TUDCA reduces CBDL-induced CHOP and pro-apoptotic markers. (a) Representative Western blot of CHOP on whole liver lysates. β-Tubulin was used for normalization. Quantification of CHOP blot compared to β-tubulin bands. Samples of three to five mice per group were mixed for blotting; (b) Real-time qPCR analysis of pro-apoptotic genes caspase 3 and 12, TNFRSf1a, FADD and Bcl2. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001; (c) Western blot and quantification of cleaved caspase 3 and cleaved caspase 12 on whole liver lysates. β-Tubulin was used for normalization. One to two samples per group were loaded with one sample consisting of a mixture of two mice; (d) Real-time qPCR analysis of pro-pyroptotic marker caspase 1. Data are presented as mean ± SD; (e) Western blot of NLRP3 on whole liver lysates. Quantification of NLRP3 was normalized to total protein load. Three samples per group were loaded with one sample consisting of a mixture of two mice, so a total of six mice were analyzed.