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. 2017 Jan 23;18(1):226. doi: 10.3390/ijms18010226

Figure 1.

Figure 1

RHPN1-AS1, a cytoplasmic lncRNA, is aberrantly expressed in UM cell lines. (A) RHPN1-AS1 expression was measured by real-time PCR in different UM cells and normal cell (RPE). RHPN1-AS1 presented higher expression in melanoma cell lines OCM1 and OM431 than RPE cells; (B) RHPN1-AS1 is cytoplasmically distributed. RNA fluorescence in situ hybridization (FISH) (red) was performed with cy3-labeled probes that recognizing RHPN1-AS1. The scale bars represent 20 µm; (C) RHPN1-AS1 is associated with the cytoplasmic fractions. Total RNAs from OCM1 cells were separated into cytoplasmic and nuclear soluble fractions. U1, GAPDH were used as controls; (D,E) The interference rate was detected 48 h after RHPN1-AS1 siRNAs transfection in OCM1 and OM431 cells. Triplicate assays were performed for each sample and the relative level of RHPN1-AS1 was normalized to the GAPDH. (* p < 0.05).