Figure 5.

P. berghei development inside Huh7 cells results in a decreased ATP/AMP ratio and increased GLUT1 content at the plasma membrane. A. Huh7 cells were transfected with an ATP probe, AT1.03, and infected with red fluorescent protein‐expressing P. berghei sporozoites. Thirty and 48 hpi, the Venus/cyan‐flourescent proteins (CFP) emission ratio in the cytoplasm of each individual cell (non‐infected and cells containing developing parasites) was calculated from the fluorescent images acquired in both channels. Huh7 cells incubated in RPMI without glucose with 10 mM of galactose and 10 μM of Oligomycin were used as positive controls for ATP depletion. Pool of 2 and 3 independent experiments for the 30 and 48 h time points, respectively. B. Huh7 cells were infected with red fluorescent protein‐expressing sporozoites and, at 30 and 48 hpi, were incubated with the H_RBD‐EGFP peptide that binds specifically to GLUT1 at the surface of the cells and were analysed by flow cytometry. Huh7 cells incubated overnight in complete RPMI with 250 μM of CoCl₂ were used as positive controls. Pool of 2 and 4 independent experiments for the 30 and 48 h time points, respectively. Error bars represent SEM. Both panels: one‐way ANOVA with post‐test Tukey. ns, not significant; * P < 0.05 and *** P < 0.001.