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. 2015 Apr 23;33(5):1419–1433. doi: 10.1002/stem.1944

Figure 3.

Figure 3

β‐Catenin enhances human embryonic stem cell (hESC) self‐renewal through upregulation of E‐cadherin. (A–C): Inducible over‐expression of E‐cadherin (E‐cad) in hESCs enhances E‐cadherin expression in cell‐cell junctions (A, immunofluorescence, left panel: enlarged images; DOX: 3 days), and increases the expression of E‐cadherin, Oct3/4, and Nanog (B, Q‐PCR, DOX: 24 hours) and cell density (C, bright‐field image and cell counting; low panel: enlarged images; DOX: 3 days). Scale bars = 10 µm (A), 20 µm (low panel of C), and 100 µm (upper panel of C); DOX, 2 µg/ml. (D, E): Reduced expression levels of E‐cadherin, Oct3/4, and Nanog (D, Q‐PCR) and diminished clonogenic capacity (E, clonogenic assays, n = 3, **, p < .01) in hESC 24 hours after E‐cadherin siRNA knockdown. H1, H9, or CA1 hESC lines were used in this figure.