Figure 3.

APAP and CCL₄ caused maximal liver damage at 24 h post‐dose. Rats received the indicated concentrations of vehicle, APAP or CCL₄ and were assessed for liver damage at 24 and 72 h post‐dose. (A) Serum ALT. (B) Serum AST. (C) Representative histological images of control and treated liver central vein sections. Black arrowheads indicate necrosis, white arrowheads indicate vacuolization; and black bar 50 μm. (D) Incidence and severity of liver microscopic findings. Findings were graded on a scale of 0 (absent), 1 (minimal), 2 (mild), 3 (moderate) or 4 (marked). ALT, alanine aminotransferase; APAP, acetaminophen; AST, aspartate aminotransferase; CCL₄, carbon tetrachloride.