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. 2017 Feb 8;12(2):e0171625. doi: 10.1371/journal.pone.0171625

Fig 2. Immunofluorescent (IF) detection of MMP-2 isoform expression in HK2 cells: effect of culture in normal (5 mM) vs. high (30 mM) glucose.

Fig 2

Panel I. IF staining for FL-MMP-2 isoform of HK2 cells cultured in normal (5 mM) glucose for 24 and 48 hours (A, C). IF staining for FL-MMP-2 isoform of HK2 cells cultured in high (30 mM) glucose for 24 and 48 hours (B, D). Upper panel x 400; lower panel x 800. Panel II. IF staining for NTT-MMP-2 isoform of HK2 cells cultured in normal (5 mM) glucose for 24 and 48 hours (A, C). IF staining for NTT-MMP-2 isoform cultured in high (30 mM) glucose for 24 and 48 hours (B, D). Upper panel x 400; lower panel x 800. Panels III and IV: Quantitation of IF staining for the FL-MMP-2 isoform (panel III) and NTT-MMP-2 (panel IV) of HK2 cells cultured in normal (5 mM) and high (30 mM) glucose medium for 24 and 48 hours. (N = 5 for all study groups; *p<0.05).