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. 2017 Feb 8;12(2):e0171479. doi: 10.1371/journal.pone.0171479

Fig 7. Eupatilin reduces lipid peroxidation in the post-ischemic brain of tMCAO-challenged mice.

Fig 7

Mice were challenged with tMCAO and eupatilin (Eup, 10 mg/kg, p.o.) was administered immediately after reperfusion. (A) Effects of eupatilin on lipid peroxidation were determined by immunohistochemistry using an antibody against 4-HNE in tMCAO-challenged brains 22 h after reperfusion. Representative images of 4-HNE-immunopositive cells in periischemic (‘P’) and ischemic core (‘C’) regions. Diagram boxes in top panels display brain areas where the images in lower panels were acquired. Dashed lines indicate the lesion site. Scale bars: 200 μm (top panels), 50 μm (middle and bottom panels). (B) Effects of eupatilin (Eup) on 4-HNE production were determined by Western blot in tMCAO-challenged brains 1 day after reperfusion. Changes in 4-HNE and α-tubulin protein levels in the hemisphere with ischemic challenge were shown as representative Western blots and quantification. n = 6 per group. **P<0.01 versus the vehicle-treated tMCAO group (tMCAO+veh).