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. 2017 Feb 8;12(2):e0170470. doi: 10.1371/journal.pone.0170470

Fig 5. HSPA9 inhibitor MKT-077 reduces cell growth, induces apoptosis, and increases p21 and BAX expression in CD34+ cells.

Fig 5

(A) Immunoblot of HSPA9, p21, BAX and TP53 following MKT-077 treatment of CD34+ cells grown in erythroid culture conditions for 5 days. (B) CD34+ cells were seeded at equal concentrations in erythroid culture conditions, and the total number of cells was counted daily in the presence of MKT-077. The fold change in cell counts was calculated relative to the number of cells on day 1 (n = 3) (0.5 μM versus control, p<0.001; 2 μM versus control, p<0.001). (C) Quantification of Annexin V+ cells (a surrogate for apoptosis) following treatment of CD34+ cells with various concentrations of MKT-077 for 5 days (n = 3). ***p<0.001.