Fig 15. Verification of NF-κB interactions with the binding sites in the Hb-α promoter by Chromatin immune precipitation assay (ChIP).

Cross-linked and sheared chromatin from hPVECs was immune-precipitated with p65- NF-κB antibody and analysed by RT-PCR. The results revealed that NF-κB efficiently interacted with the binding sites present in the Hb-α promoter (a: untreated; b: LPS treated (10 μg/ml for 2 hrs); c: LPS treated for 24 hrs; 1: Input sample from control; 2: p65- NF-κB antibody pull down from control and LPS treated (b,c); 3: IgG antibody pull down from control (a) and LPS treated (b, c).