Table 1.

Subjects.

Histochemical Staining Measure	Number of Subjects	Males	Females	TTF Range
NeuN cell #	26	11	15	0.04–8.25
PV cell #	26	11	15	1.82–10.03
Orexin-A cell #	36	18	18	0.15–6.81
BrdU cell #	16	16	0	0.32–3.79
DCX cell #	16	16	0	0.32–3.79
proBDNF+ cell #	36	19	17	0.43–7.65
proBDNF+ puncta #	36	19	17	0.43–7.65
proBDNF OD in GM	36	19	17	0.43–7.65
proBDNF OD in WM	36	19	17	0.43–7.65
dMBP fluorescence intensity	29	13	16	1.15–10.89
HA percent area	29	13	16	1.15–10.89

The number of subjects and range of freezing times (TTF) are shown for each cohort. Some staining measures were acquired as double labels and, thus, share a cohort and/or TTF range. These include NeuN and PV (same cohort, separate experiments), BrdU and DCX (same cohort, separate experiments), dMBP and HA (double label; same cohort and TTF range), and all proBDNF stains (different analysis measures derived from one batch of immunostained tissue). Abbreviations: TTF, total time frozen; NeuN, neuronal nuclei; PV, parvalbumin; BrdU, bromodeoxyuridine; DCX, doublecortin; proBDNF, pro-brain-derived neurotrophic factor; OD, optical density; GM, gray matter; WM, white matter; dMBP, damaged myelin basic protein; HA, hyaluronic acid.