Figure 6.

Reduced levels of aggrecan (Acan) in collagen XIX-deficient cerebral cortex and hippocampus. Western blots demonstrate reduced levels of Acan in protein extracts derived from P67 control (Ctl) or col19a1^−/− mutant (KO) cortex (a), hippocampus (HP; (c)), and cerebellum (Cblm; (e)). Levels of Actin protein were used as loading controls. Quantification of levels of Acan detected by Western blot and normalized to Actin levels. Data shown are mean relative intensities normalized to Actin ± SD (n = 3). * indicates differs from littermate WT by p < 0.05 by Student’s t-test. ** indicates differs from littermate WT by p < 0.0001 by Student’s t-test.