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. 2017 Jan 1;9(1):1759091416689020. doi: 10.1177/1759091416689020

Figure 9.

Figure 9.

Ectopic neuronal expression of adamts4 mRNA and increased active ADMATS4 peptides in the absence of collagen XIX. ((a) and (b)) In situ hybridization for adamts4 and syt1 mRNA in adult control or col19a1−/− mutant cortex (Ctx) and hippocampus (HP). Note the co-expression of adamts4 and syt1 mRNA in neurons in mutant Ctx and HP. ((c) and (d)) In situ hybridization for adamts4 and IHC for NeuN in adult control or col19a1−/− mutant cortex (Ctx) and hippocampus (HP). Note the ectopic co-expression of adamts4 and NeuN in neurons in mutant Ctx and HP. (e) Western blots of ADAMTS4 in protein extracts from control or col19a1−/− mutant Ctx. The pro- and active forms of ADAMTS4 are indicated with arrows (pro-form – 98 kDa; active form – 64 kDa). (f) Quantification of amounts of pro- and active forms of ADAMTS4 in control and col19a1−/− mutant Ctx. Data shown are mean relative intensities (normalized to actin) ± SD (n = 3). * indicates differ from controls by p < 0.05 by Student’s t-test. Scale bar = 20 µm.