Figure 1.

C5aR1 expression on primary neurons. Primary neurons from WT mice and C5aR1KO mice were generated using E15-E16 pups, cultured for 7 to 10 days, and RNA was collected from either (a) unstimulated cultures (WT n = 4; KO n = 2) or from (b) cultures treated with 0, 10, or 100 ng/ml of TNF-α for 24 hr (n = 2 for WT cells and n = 1 for C5aR1 knock out cells). Quantitative RT-PCR for C5aR1 expression relative to HPRT was performed with technical triplicates in each experiment. p values are calculated using unpaired two-tailed t test. Values of p < .05 were considered statistically significant.