Figure 3.

C5a increases toxicity in fAβ-treated neurons. Primary neurons from WT mice (a–e and k) or C5aR1KO mice (f–j and l) were generated using E15-E16 pups and cultured for 7 to 10 days. The cells were then stimulated or not with 5 µM fAβ, 100 nM hC5a, and 100 nM PMX53 for 24 hr. MAP-2 was visualized by immunocytochemistry (20 × magnification) and quantified using ImageJ software (k, l). Data are presented as mean MAP-2 area ± SEM of all image values from n = 3 independent experiments, each with three coverslips per treatment, five images per coverslip. p values are calculated using unpaired two-tailed t test. Values of p < .05 were considered statistically significant.