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Primary neuronal cultures include GABAergic and glutamatergic neurons. Immunofluorescent staining of GAD67 (red, GABAergic neurons; left panel) or VGLUT1 (red, Glutamatergic neurons; right panel) in 7-day mouse neuron cultures. Nuclei are labeled with DAPI (blue; 10× magnification). Top right Inset in each panel is 20× magnification of region denoted by the arrow. Scale Bar: 50 µm.
