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. 2016 Nov 5;242(4):441–447. doi: 10.1177/1535370216677354

Figure 4.

Figure 4

Suppression of ER stress elevates adiponectin expression in tunicamycin-induced 3T3-L1 adipocytes. Pretreatment of 5 µg/mL tunicamycin (Tun) for 4 h was used to induce ER stress in 3T3-L1 adipocytes. The cells were cultured in the presence or absence of tunicamycin with or without 1 µg/mL FGF21 or 1 mmol/L TUDCA for 4 h. For insulin signaling, cells were stimulated with 10 nmol/L of insulin for 10 min. (a) Protein expression of adiponectin, ATF4, CHOP and phosphorylation of eIF2α in adipocytes. (b) The relative protein quantity of adiponectin (Adpn), ATF4, CHOP and phosphorylation of eIF2α in adipocytes. (c) Adiponectin in the medium. (d) Phosphorylation of IRS-1 and Akt in adipocytes. (e) The relative protein quantity of p-IRS-1 and p-Akt in adipocytes. (f) Glucose uptake. The relative quantity of proteins was analyzed using Quantity One software. A representative blot is shown and the data were expressed as mean ± SEM in each bar graph. *P < 0.05 (Tun versus Veh). #P < 0.05 (Tun/FGF21 or TUDCA versus Veh)