Figure 13.

Schematic representation of the analytical protocol used to detect immunoglobulin IgY, extracted from tempera paints. MB and LB are the oxidized and reduced forms of the redox mediator methylene blue and HRP is horseradish peroxidase, used as enzymatic label for the anti-IgY antibody (a) capture of immunoglobulin IgY on the polycarbonate of the NEE; (b) molecular recognition by anti-IgY labeled with HRP; (c) generation of the electrocatalytic cycle by adding the enzyme substrate H₂O₂ and the mediator MB. (reprinted with permission from [109]).