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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Neuroreport. 2017 Mar 1;28(4):208–213. doi: 10.1097/WNR.0000000000000746

Figure 2. Polyamines prevented uncoupling of Novikoff cells induced by enhanced intracellular calcium or hydrogen concentrations.

Figure 2

(A) Examples of junctional currents (Ij) records in response to repeated (0.7 Hz) Vj ramps shown in (B). (C) Changes in the time course of averaged and normalized gj at different [Ca2+]i and spermine (SPM). An increase of [Ca2+]i from 20 nM to 1 μM accelerated gj decay (filled circles over filled squares). Addition of SPM at 0.1 mM (up triangles), 1 mM (down triangles) and 10 mM (diamonds) decelerated gj decay up to full recovery at 10 mM of SPM. (D) A summary of data demonstrating a lessening of Ca2+-induced uncoupling by SPM (p<0.05, n=7 in each group). (E) Averaged and normalized gj show that spermidine (SPD) diminish acidosis-mediated gj decay in a concentration dependent manner (p<0.05, n=6 in each group). Data shown in grey columns (SPM) demonstrate that SPD effect is lower than that of SPM at all three concentrations. (F) Dose-response relationships of normalized gj recovery under an influence of SPM and SPD. Curves are fits of a Logistic equation to the experimental data.