|
REAGENT or RESOURCE |
SOURCE |
IDENTIFIER |
|
Antibodies |
|
Goat polyclonal anti-GluA3 |
Santa Cruz Biotechnology |
Cat#: sc7613 |
|
Mouse monoclonal anti-Gephyrin |
Synaptic Systems |
Clone mab3b11, cat#147 111 |
|
Mouse monoclonal anti-GABAAα1 |
Millipore |
Clone mab339, cat# 2090441 |
|
Rabbit polyclonal anti-GABACρ |
R. Enz/H. Wassle |
N/A |
|
Donkey polyclonal anti-goat Alexa Fluor 647 |
Jackson ImmunoResearch |
Code: 705-605-147 |
|
Donkey polyclonal anti-mouse Alexa Fluor 488 |
Jackson ImmunoResearch |
Code: 715-545-150 |
|
Donkey polyclonal anti-rabbit Alexa Fluor 488 |
Jackson ImmunoResearch |
Code: 711-545-152 |
|
Donkey polyclonal anti-mouse Alexa Fluor 647 |
Jackson ImmunoResearch |
Code: 715-605-150 |
|
Streptavidin Alexa Fluor 568 conjugate |
Invitrogen molecular probes |
Cat#: S11226
|
|
Biological Samples |
|
Macaque retina |
Regional Primate Research Centre |
N/A |
|
Chemicals, Peptides, and Recombinant Proteins |
|
Ames |
Sigma |
1420 |
|
GABAzine |
Sigma |
SR-95531 |
|
TPMPA |
Tocris |
1040 |
|
Strychnine |
Sigma |
S8753 |
|
MNI-glutamate |
Tocris |
1490 |
|
RuBi-GABA |
R&D Systems |
3400 |
|
NBQX |
Tocris |
1044 |
|
Neurobiotin |
Vector Labs |
SP1120 |
|
Gold Particles/micro carriers (1.6μm diameter) |
Bio-Rad |
Cat#: 165-2264 |
|
Vectashield Antifade mounting medium |
Vector Labs |
CatN: H-1000 |
|
GTPγS |
Sigma |
G8634 |
|
Recombinant DNA |
|
pcDNA-tdTomato |
R. Wong, University of Washington Morgan et al., 2008 |
N/A |
|
pcDNA-PSD95CFP |
Construct modified from PSD95-YFP construct from A.M. Craig, University of British Columbia Kerschensteiner et al., 2009 |
N/A |
|
pcDNA-PSD95YFP |
A.M. Craig, University of British Columbia Morgan et al., 2008 |
N/A |
|
pcDNA- GABAAα1-YFP |
This paper (construct modified from GABAAα1-pHGFP construct from T. Jacobs, University of Pittsburgh) |
N/A |
|
pcDNA- GABAAγ2 |
A.M. Craig, University of British Columbia |
N/A |
|
pcDNA- GABAAβ2/3 |
A.M. Craig, University of British Columbia |
N/A |
|
Software and Algorithms |
|
IGOR Pro |
WaveMetrics |
https://www.wavemetrics.com/ |
|
MATLAB |
Mathworks |
http://ch.mathworks.com/products/matlab |
|
Symphony |
Symphony-DAS |
https://github.com/symphony-das |
|
ImageJ NIH |
|
https://imagej.nih.gov/ij/ |
|
Amira FEI |
Visualization Sciences Group |
https://www.fei.com/software/amira-3d-for-life-sciences/ |
|
MetaMorph |
Molecular Devices |
https://www.moleculardevices.com/ |
|