Effect of fasting on triglyceride levels, the abundance of HSL and its inhibitory phosphorylation at Ser-565 in hearts of WT and GC+/0 mouse hearts. Data are means±SEM of 3-5 freeze-clamped hearts. (A) Triglyceride content of WT (solid bars) and GC+/0 (open bars) mouse hearts, either fed or following a 24-h fast (fasted). (B) HSL protein, normalized to GAPDH, and (C) phosphorylation levels of HSL (Ser-565), normalized against total HSL protein, in fasted WT and GC+/0 mouse hearts. Western blot analyses were as described in Fig. 4. *P < 0.05 GC+/0 vs WT hearts.