. Author manuscript; available in PMC: 2017 Feb 8.

Published in final edited form as: J Mol Cell Cardiol. 2008 May 27;45(2):230–239. doi: 10.1016/j.yjmcc.2008.05.012

Table 2.

Metabolic parameters of GC^+/0 hearts and their WT littermates

Parameters	WT	GC^+/0
OLE/CS	0.49±0.03	0.31±0.05^*
PDC/CS	0.59±0.07	0.52±0.04
PC/CS	0.05±0.01	0.04±0.01
Contribution of carbohydrates to tissue pyruvate formation (%)
Glucose	32±4%	33±2%
Pyruvate+lactate	54±3%	54±1%
Others	18±4%	13±3%
Malonyl-CoA concentration (nmol/gww)	1.16±0.04	1.23±0.07
ACC activity (μmol min⁻¹ gww⁻¹)	0.76±0.12	0.83±0.09
[Acetyl-CoA]-to-[CoA] ratio (arbitrary units)	0.0559±0.0054	0.0580±0.0073
Citric acid cycle flux rate (μmol min⁻¹ gww⁻¹)	2.85±0.20	3.11±0.08

Data are means±SEM of 3–8 heart perfusion experiments. The Experimental procedures provide details about the (i) determinations of flux ratios, which reflect the contribution exogenous fatty acids (oleate) and of carbohydrates (lactate, pyruvate and glucose) to acetyl-CoA and or oxaloacetate formation for citrate synthesis (CS), and (ii) calculation of citric acid cycle flux rate, and (iii) determinations of levels of CoA derivatives and acetyl-CoA carboxylase (ACC) activity. OLE: oleate oxidation; PDC: pyruvate decarboxylation; PC: pyruvate carboxylation.

P < 0.05.