Figure 5.

The morphology of oospores at different developing stages. The oospores from WT, CK, and PiLLP-silenced transformants (STs) were observed with light microscope before (A) or after (B) they were treated with tetrazolium bromide (MTT), or were observed with Scanning Electronic Microscope (C). Oospores from 7, 11, and 17 days old cultures were used and those stained red were considered to be viable, whereas the unstained ones were nonviable. Oospores in (C) were from 11 days old cultures. The bars indicate 10 μm. (D) Development of oospores. At the early stage of oospore development, oogonial wall begin to thick, and then oospore was formed, finally the mature oospore was produced, consisting of oospore wall, lipid body and ooplast (Drawing according to Hemmes, 1983). (E) The distribution of lipid body in oogonia at different developing stages. Oogonia from 7, 11, and 17 days old cultures of WT, CK, and PiLLP-silenced transformants (STs) were treated with Nile red and observed under fluorescence microscope. The red emission indicated the distribution of lipid body. The bars indicate 10 μm.