Figure 6.

Asexual phenotype characterization. (A) Growth of the WT, CK, and the PiLLP transformants (S3, S16, S84, O36, and O38). Photos were taken at 7 days after incubation on 10% TRA medium. (B) Statistics of colony diameters was based on 7 days of growth on 10% TRA medium. (C) Sporangia of the WT, CK, and the PiLLP transformants (S3, S16, S84, O36, and O38) formed on 10 days old cultures were washed with water and then counted under the microscope. (D) Sporangia suspension was incubated in a plate for 2 h at 4°C. The ratios of the number of empty sporangia to the total number of sporangia were calculated for each strain. (E) Sporangia suspension was incubated in 200 g/L Peabroth at 18°C. The numbers of germinated and un-germinated sporangia were counted under a microscope at different time points (0, 1.5, 3, 4.5, 6, and 7.5 h), and the percentage of the germinated sporangia to the total number of sporangia were calculated for each strain. Three replicates were used for each treatment and the whole experiment was repeated once with three replicates. Bars represent the standard deviation of three replicates. Statistical significance was analyzed with Student's t-test between WT and each transformant (^*P < 0.05, ^**P < 0.01).