Figure 8.

PiLLP participates in the response to oxidative stress. (A) Levels of PiLLP expression in mycelia grown on medium amended with various concentration of hydrogen peroxide (H₂O₂). The qRT-PCR assay was performed using cDNAs synthesized from mycelial RNAs. The level of PiLLP expression was determined relative to that of ef1, and the relative gene expression levels shown were determined relative to expression on TRA medium alone (value, 1.0). (B) Colony expansion of WT, CK, and PiLLP transformants (S3, S84, O36, and O38) on TRA medium supplemented with 0, 0.5, 1.0, 1.5, and 2.0 mM H₂O₂. (C) Colony diameters were measured after 7 days of growth. Rates of growth inhibition were calculated for each isolates of each treatment relative to the growth on TRA medium. Three replicates were used for each treatment in these tests and the whole experiment was repeated once. Bars represent the standard deviation of three replicates. Statistical significance was analyzed using Student's t-test between WT and each transformant (^*P < 0.05, ^**P < 0.01).