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. 2017 Feb 9;8:142. doi: 10.3389/fpls.2017.00142

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Copyright © 2017 Guo, Wang, Shan, Sun and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Infection assay on detached potato leaves. (A) Leaves of potato cultivar Impala were inoculated with sporangia (~100 in 10 μL for WT, CK, and PiLLP-overexpressed sporangia, and 200 for PiLLP-silenced transformant sporangia). Images were taken at 5 day after inoculation. (B) Sizes of lesions induced by different isolates. The diameter of each lesion was measured at 5 day after inoculation. Three replicates were used for each treatment in these tests and the whole experiment was repeated once. Bars represent the standard deviation of three replicates. Statistical significance was analyzed using Student's t-test (^**P < 0.01). (C) The amplified image of inoculated leaves with different isolates (WT, S84, and O36).