FIGURE 2.

Isolation of extracellular vesicles from plasma. (A) ECVs were extracted from 0.5 ml plasma obtained from F0/F1/F2 patients with chronic HBV and HCV infection, and a healthy control group. After centrifugation, a vesicle-rich pellet is obtained, which is dissolved in PBS for further analysis. (B) Obtained vesicle-suspensions were analyzed by western blot for expression of the vesicle-markers CD63 and TSG101, and the absence of the cellular marker calreticulin. (C) Protein content of ECVs isolated from different groups. An increase in protein concentration can be seen comparing vesicle-preparations of healthy subjects with HBV and HCV patients. ^∗∗P < 0.01.