Table 2.
miRNA | Mature miRNA primer |
---|---|
miR-92a-3p | TATTGCACTTGTCCCGGCCTGT |
miR-122-5p | TGGAGTGTGACAATGGTGTTTG |
miR-150-5p | TCTCCCAACCCTTGTACCAGTG |
miR-192-5p | CTGACCTATGAATTGACAGCC |
miR-200b-3p | TAATACTGCCTGGTAATGATGA |
miR-21-5p | TAGCTTATCAGACTGATGTTGA |
cel-miR-39-3p | AGCTGATTTCGTCTTGGTAATA |
All primers were ordered from Integrated DNA Technologies (IDT, Leuven, Belgium), and are specific for detection of both human and murine miRNA-expression. Lyophilized primers were dissolved in RNase-free water to obtain primer solutions with a final concentration of 10 pmol/μl.