Table 3.

Messenger RNA (mRNA) primers.

Gene	Messenger RNA (mRNA) primer
Gapdh	FP: TCGAGATCGCCACCTACAG
	RP: GTCTGTACAGGAATGGTGATGC
Lox	FP: CTCCTGGGAGTGGCACAG
	RP: CTTGCTTTGTGGCCTTCAG
Col1a1	FP: ACCTAAGGGTACCGCTGGA
	RP: ACCTAAGGGTACCGCTGGA
Acta2	FP: CCAGCACCATGAAGATCAAG
	RP: TGGAAGGTAGACAGCGAAGC

All primers were ordered from Integrated DNA Technologies (IDT, Leuven, Belgium), and are specific for detection of murine mRNA-expression. Lyophilized primers were dissolved in RNase-free water to obtain primer solutions with a final concentration of 10 pmol/μl. FP, forward primer; RP, reverse primer.