Figure 7.

5-HT-induced mitochondrial ${{\text{O}}_2}^{\underset{\_}{•}}$ production is sensitive to extracellular calcium and rotenone in rat PASMC. (A) Shows images of mitochondrial mitosox fluorescence in untreated conditions (in presence or absence of extracellular calcium, Aa and Ac respectively) and following a 60 min incubation with 5-HT (100 μM) in the presence or absence of extracellular calcium (Ab and Ad respectively). Scale bar is 20 μm for all images. (B) Means ± SEM of the percent change in mitosox fluorescence after 60 min in untreated conditions in the presence of extracellular calcium or not (white and dark gray columns respectively), following 60 min of 5-HT (100 μM) incubation in the presence of extracellular calcium or not (black and light gray columns respectively), in unstimulated conditions with rotenone (100 nM) (hatched column) and finally following a 60 min incubation with 5-HT, rotenone and both intra and extracellular calcium (double hatched column). n is the number of cells tested. ^* and ^** indicate a significant difference for P < 0.05 and P < 0.01 respectively.