Table 2.

Differential abundance analysis exhibiting OTUs strongly influenced by MNPK (manure plus NPK), SNPK (straw plus NPK), and HNPK (high rate of N, regular PK) treatments, respectively.

	Enriched OTUs					Depleted OTUs
	Phylum level	Count	Most influential OTUs	Log2 FC	P-adjusted	Phylum level	Count	Most influential OTUs	Log2 FC	P adjusted
MNPK vs. CK	Unclassified	46	Arenimonas	2.433	<0.001	Unclassified	91	Gaiella	−1.763	<0.001
	Gammaproteobacteria	28	Arenimonas	2.928	<0.001	Acidobacteria	34	Gaiella	−2.070	0.004
	Bacteroidetes	22	Arenimonas	2.755	<0.001	Alphaproteobacteria	21	Nitrospira	−3.079	0.002
	Acidobacteria	18	Gemmatimonas	2.971	<0.001	Actinobacteria	16	Nitrospira	−5.417	<0.001
	Betaproteobacteria	18	Gemmatimonas	5.020	<0.001	Bacteroidetes	16	Unclassified Anaerolineaceae	−4.012	0.007
	Gemmatimonadetes	11	Lysobacter	1.829	<0.001	Deltaproteobacteria	12	Unclassified Chitinophagaceae	−3.976	<0.001
	Deltaproteobacteria	6	Unclassified Xanthomonadaceae	4.051	<0.001	Planctomycetes	10	Unclassified Comamonadaceae	−1.573	0.011
	Verrucomicrobia	4	Unclassified Xanthomonadaceae	2.714	<0.001	Verrucomicrobia	10	Unclassified Rhodospirillaceae	−2.411	<0.001
	Others	10	Unclassified Xanthomonadaceae	2.545	<0.001	Others	38	Unclassified Sphingomonadaceae	−2.647	<0.001
	Total	163	Unclassified Xanthomonadaceae	5.114	<0.001	Total	248	Unclassified Sphingomonadaceae	−2.355	0.002
SNPK vs. CK	Unclassified	42	Arenimonas	2.352	<0.001	Unclassified	46	Gaiella	−1.754	0.009
	Bacteroidetes	20	Arenimonas	2.720	0.001	Acidobacteria	19	Gaiella	−3.369	<0.001
	Gammaproteobacteria	12	Arenimonas	3.427	<0.001	Alphaproteobacteria	14	Rhodospirillales mumbers	−2.797	<0.001
	Betaproteobacteria	10	Aureispira	3.111	0.004	Actinobacteria	9	Nitrospira	−4.468	<0.001
	Acidobacteria	9	Gemmatimonas	3.011	<0.001	Bacteroidetes	8	Sphingomonas	−1.886	0.032
	Deltaproteobacteria	6	Gemmatimonas	2.447	0.030	Gammaproteobacteria	5	Unclassified Chitinophagaceae	−6.649	0.002
	Gemmatimonadetes	5	Gemmatimonas	4.348	0.006	Verrucomicrobia	5	Unclassified Rhodospirillaceae	−2.024	0.027
	Verrucomicrobia	2	Unclassified Cryomorphaceae	3.691	0.022	Deltaproteobacteria	4	Unclassified Sphingomonadaceae	−2.410	0.002
	Others	2	Unclassified Xanthomonadaceae	2.170	0.022	Others	16	Unclassified Sphingomonadaceae	−2.588	0.002
	Total	108	Unclassified Xanthomonadaceae	5.266	<0.001	Total	126	Unclassified Sphingomonadaceae	−3.082	0.047
HNPK vs. CK	Unclassified	17	Arenimonas	2.200	0.001	Unclassified	55	Gaiella	−1.833	0.001
	Bacteroidetes	14	Cellvibrio	2.618	0.001	Bacteroidetes	11	Gaiella	−1.628	0.002
	Gammaproteobacteria	10	Gemmatimonas	2.059	<0.001	Actinobacteria	10	Gaiella	−2.154	0.007
	Acidobacteria	9	Lacibacter	2.590	0.009	Acidobacteria	9	Gaiella	−1.782	0.012
	Gemmatimonadetes	4	Luteolibacter	1.842	0.014	Gammaproteobacteria	6	Nitrospira	−6.931	<0.001
	Verrucomicrobia	4	Sphingomonas	1.389	<0.001	Verrucomicrobia	5	Unclassified Chitinophagaceae	−5.330	<0.001
	Alphaproteobacteria	3	Unclassified Chitinophagaceae	2.327	<0.001	Betaproteobacteria	4	Unclassified Comamonadaceae	−1.624	0.007
	Betaproteobacteria	3	Unclassified Chitinophagaceae	1.969	0.012	Deltaproteobacteria	4	Unclassified Planctomycetaceae	−4.833	0.028
	Others	5	Unclassified Xanthomonadaceae	2.953	0.006	Others	19	Unclassified Rhodocyclaceae	−4.743	0.036
	Total	69	Unclassified Xanthomonadaceae	5.321	<0.001	Total	123	Unclassified Rhodospirillaceae	−1.489	0.029