Table 2.
Primers and probes for REBA-EAC and qPCR.
| Target and primer/probe name | Nucleotide sequence (5′-3′) | Product size |
|---|---|---|
| ESBLs | ||
| CTX-M-1-210-F | GATGTGCAGCACCAGTAAAGTGATG | 203 bp |
| CTX-M-1-413-R | ATCGCCACGTTATCGCTGTACTG | |
| CTX-M-1/2-258-P | AAGTGAAAGCGAACCGAATCTGTTA | |
| CTX-M-9-210-F | TGTGCAGTACCAGTAAAGTTATGGCG | 170 bp |
| CTX-M-9-380-R | GCGCTAAGCTCAGCCAGTGACA | |
| CTX-M-9-261-P | GAAACGCAAAAGCAGCTGCTT | |
| TEM-520-F | TGCTCACCCAGAAACGCTGGT | 705 bp |
| TEM-1225-R | ACGATACGGGAGGGCTTACCAT | |
| Gln39Lys-P | AAAGATGCTGAAGATAAGTTGGGTG | |
| Glu104Lys-P | CCAACTATCAAGGCGGGTTACA | |
| Gly164His-P | TTCCCAATGATCAAGGCGAGTTA | |
| Gly238Ser-P | GAGATCCATGCTCACCGGCTC | |
| Glu240Lys-P | GAGATCCATGCTCACCGGCTC | |
| TEM-127-Fa | GGTTACATCGAGCTGGATCTCAACA | 360bp |
| TEM-488-Ra | CAACGATCAAGGCGGGTTACAT | |
| SHV-243-F | TATTATCTCCCTGTTAGCCACCCT | 717 bp |
| SHV-960-R | CCAAGCAGGGCGACAATCC | |
| Leu35Gln-P | GGCTTTCGCTTTGTTTAATTTGCTC | |
| Asp179Ala-P | GCCCGCACCACCACTA | |
| SHV-147-Fb | GCAAATTAAACAAAGCGAAAGCCA | 133bp |
| SHV-280-Rb | GCACTACTTTAAAGGTGCTCATCATGG | |
| AmpC β-LACTAMASES | ||
| ACT-MIR-416-F | TACAGGTRCCGGATGAGGTCACG | 205 bp |
| ACT-MIR-620-R | CTATGGTCCAGCTTGAGCGGCTTAA | |
| ACT-MIR-464-P | ATCAAAACTGGCAGCCGCAG | |
| CMY-2-like-261-F | AAGACGTTTAACGGCGTGTTGG | 175 bp |
| CMY-2-like-436-R | TAACGTCATCGGGGATCTGCA | |
| CMY2-like-328-P | ACGAAATACTGGCCAGAACTGACA | |
| DHA-261-F | TTTCACAGGTGTGCTGGGTGC | 200 bp |
| DHA-460-R | AGAAATTCAGCAGATCCGCACGG | |
| DHA-299-P | AAGAGATGGCGCTGAATGATCC | |
| ACC-1-410-F | ACGT AGCGTAACAAGTCGCTGGAG | 210 bp |
| ACC-1-620-R | TGGGTGTGAGCTATGAAGATGCGATT | |
| ACC-1-439-P | GCTTCGTTACCCAAAATCTCCATATTC | |
| CMY-1-like/MOX-128-F | CTGCTCAAGGAGCACMGGATC | 243 bp |
| CMY-1-like/MOX-371-R | CGGATCCCTTGAGCCAGGG | |
| CMY-1-like/MOX-172-P | AAAGGATGGCAAGGCCCACTA | |
| FOX-182-F | ATTTCAACTATGGGGTTGCCAACC | 208 bp |
| FOX-390-R | AAGCTCGGCCATRGTCACACCATC | |
| FOX-239-P | TGTTCGAGATTGGCTCGGTCAG | |
| CARBAPENEMASES | ||
| IMP-263-F | TAAAAGGCAGTATYTCCTCWCATTT | 232 bp |
| IMP-495-R | CCAAACCACTACGTTATCTKGAG | |
| IMP-307-P | GGAATAGAGTGGCTTAATTCTCRATCTAT | |
| VIM-136-F | CTTTACCAGATTGCCGATGGTGTT | 214 bp |
| VIM-350-R | TCATGAAAGTGCGTGGAGACTGCA | |
| VIM-198-P | CTACCCGTCCAATGGTCTCATTGT | |
| KPC-269-F | TGGACACACCCATCCGTTACG | 211 bp |
| KPC-480-R | ACGGAACGTGGTATCGCCGATAG | |
| KPC-329-P | AATATCTGACAACAGGCATGACGGT | |
| NDM-327-F | AGATCCTCAACTGGATCAAGCAGG | 223 bp |
| NDM-550-R | TGCTGGTTCGACCCAGCCATTGGC | |
| NDM-385-P | TCACGCGCATCAGGACAAGA | |
| OXA-48-like-131-F | TTGTGCTCTGGAATGAGAATAAGCAG | 210 bp |
| OXA-48-like-340-R | CGGTGATTAGATTATGATCGCA | |
| OXA-48-186-P | GAACCAAGCATTTTTACCCGCA | |
| SPM-50-F | CGGATCATGTCGACTTGCCCT | 200 bp |
| SPM-250-R | ACTACTTTCTTCGGCTTCATAGTC | |
| SPM-107-P | TCGTCACAGACCGCGATTTCT | |
a,b
Primers for qPCR.