Figure 3.

Effects of Cu²⁺ on Zn²⁺-induced neurotoxicity. Various concentrations of CuCl₂ (0~20 μM) without ZnCl₂ (A) or with 10 μM (B), 20 μM (C), or 30 μM ZnCl₂ (D) were administered to GT1-7 cells. After 24 h, cell viability was determined using the WST-8 method. Six wells were exposed to the same experimental conditions (n = 6). Data are presented as means ± SD. Experiments were replicated at least two times. ^*p < 0.05, ^**p < 0.01 vs. CuCl₂ (0 μM).