Figure 6.

Cytokine-targeted treatment during coculture of primary human acute myeloid leukemia (AML) cells and bone marrow mesenchymal stem cells (MSCs); a comparison of patients with different karyotype (A) and patients with and without NPM1 mutations. (A) Proliferative AML cell responses were compared for cocultures prepared with an IL-6 specific antibody and control antibody. The study included patients with normal AML cell karyotype (n = 15), favorable (n = 5), intermediate (n = 4), and adverse (n = 7) cytogenetics. The results for individual patients are presented as the proliferative response in cultures with anti-IL-6 relative to the proliferation in control cultures. The IL-6-specific antibody decreased the proliferation only for patients with normal cytogenetics but not for the other patients (Wilcoxon’s test for paired samples). (B) AML cell proliferation was also compared for patients with (gray columns) and without NPM1 mutations (white columns). Decreased AML cell proliferation was seen for several cytokine-directed treatment, especially chemokine-directed interventions (Wilcoxon’s test for paired samples for IL-6/bFGF, χ² likelihood analyses for the chemokines). The box-plots show the median value, the 25 and 75% quartiles and the variation range (minimum to maximum value).