Table 1.
Structural parameters derived from SAXS data
Rg (Å) Guinier |
Rg (Å) GNOM |
Dmax (Å) |
MWPred (kDa)a |
MWsaxs (kDa)b |
χ / χ2free | |
---|---|---|---|---|---|---|
Post-cleavage | 40.1 ± 0.5 | 40.2 ± 0.2 | 140 ± 5 | 128 | – | 1.01/1.51 |
Apo-Rnt1p | 46.7 ± 0.8 | 45.9 ± 0.5 | 160 ± 5 | 108 | 106 | 0.34/0.12 |
The MWpred was calculated from the primary sequences of components.
The MWSAXS was calculated using the power-law relationship between a mass parameter, QR, and particle mass, empirically determined on the basis of SAXS data for a particular class of macromolecular particles. Due to the lack of SAXS data, such relationship remains undetermined for protein-nucleic acid complexes (Rambo and Tainer, 2013a).