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. Author manuscript; available in PMC: 2018 Feb 7.
Published in final edited form as: Cell Metab. 2017 Jan 12;25(2):358–373. doi: 10.1016/j.cmet.2016.12.010

Figure 1. High-fat diet selectively promotes tumor growth potential of BRAF V600E-positive melanoma cells in xenograft nude mice.

Figure 1

(A) Tumor growth (left), weight (middle) and body weight (right) of xenograft nude mice injected with human melanoma BRAF V600E-positive A375 (upper panels), or SK-MEL-2 cells (NRAS Q61K; lower panels) that were fed with normal diet, or different high-fat diets. Data are mean ± SEM for tumor growth and mean ± s.d. for tumor weight; p values were obtained by a two-way ANOVA test for tumor growth rates and a two-tailed Student’s t test for tumor masses.

(B–D) Acetoacetate (AA; B), β-hydroxybutyrate (3HB; C), and cholesterol (D) levels in serum harvested from A375 and SK-MEL-2 xenograft mice fed with normal or different high-fat diets. Data are mean ± s.d.; n=3; p values were obtained by a two-tailed Student’s t test.

(E–F) Western blot results show MEK1 and ERK1/2 phosphorylation (E) and BRAF-MEK1 binding (F) in tumor tissue samples obtained from xenograft mice.

(G) Summarized results of immunohistochemical (IHC) staining assay detecting Ki67-positive cells in tumor tissue samples from A375 and SK-MEL-2 xenograft mice. Data are mean ± s.d.; p values were obtained by a two-tailed Student’s t test.

Also see Figure S1.