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. Author manuscript; available in PMC: 2018 Feb 7.
Published in final edited form as: Cell Metab. 2017 Jan 12;25(2):358–373. doi: 10.1016/j.cmet.2016.12.010

Figure 2. Intraperitoneally injected acetoacetate selectively promotes BRAF V600E-positive melanoma tumor growth.

Figure 2

(A) Tumor growth (left) and weight (right) of xenograft nude mice injected with human melanoma BRAF V600E-positive A375 (upper panels) or HMCB (NRAS Q61K; lower panels) cells that were intraperitoneally injected with AA or 3HB. Data are mean ± SEM for tumor growth and mean ± s.d. for tumor weight; p values were obtained by a two-way ANOVA test for tumor growth rates and a two-tailed Student’s t test for tumor masses.

(B–C) AA (B) and 3HB (C) levels in serum harvested from A375 and HMCB xenograft mice treated with AA or 3HB. Data are mean ± s.d.; n=3; p values were obtained by a two-tailed Student’s t test.

(D–E) Western blot results show MEK1 and ERK1/2 phosphorylation (D) and BRAF-MEK1 binding (E) in tumor tissue samples obtained from xenograft mice.

(F) Summarized results of IHC staining assay detecting Ki67-positive cells in tumor tissue samples from xenograft mice. Data are mean ± s.d.; p values were obtained by a two-tailed Student’s t test.

Also see Figure S2 and S3.