Relationship between the number of antigens recognized and the IgG antibody signal. Serum samples taken on 18 March 2014 (n = 447) were analyzed for the number of antigens recognized by antibody and the RLU signal obtained for each antigen individually. *, P < 0.01; **, P < 0.001; ***, P < 0.0001; NS, not significant; pep, peptide.