TABLE 3.
IgG antibody levels above the cutoff in serum samples from the index herd in relation to skin test status
| Blood sample datea and skin test status | Relative light units × 10−3 (mean ± SD)b |
|||||
|---|---|---|---|---|---|---|
| MPB70 peptide | PPD-B | MPB70 | MPB83 | CFP10 | ESAT6 | |
| 18 March 2014 | ||||||
| SICTT positive | 48.1 ± 7.6 | 46.4 ± 6.1 | 49.4 ± 5.4 | 43.3 ± 8.2 | 40.5 ± 12.6 | 44.2 ± 11.8 |
| SICTT negative | 30.4 ± 13.4c | 24.0 ± 13.6c | 32.7 ± 13.0c | 26.8 ± 11.0c | 24.4 ± 11.7c | 26.1 ± 14.6c |
| 22 May 2014 | ||||||
| SIT positive | 25.8 ± 14.0 | 16.4 +/12.7 | 26.4 ±12.3 | 26.8 ± 11.9 | 25.2 ± 13.8 | 29.3 ± 13.1 |
| SIT negative | 20.8 ± 12.8 | 12.3 ± 7.3 | 21.0 ± 9.5d | 22.4 ± 11.3 | 19.8 ± 8.1 | 24.6 ± 10.9 |
a
SICTT was performed in March and 447 blood samples were taken 8 days after PPD-B injection, while SIT was performed in May and 207 blood samples were taken 3 days after PPD-B injection. Only RLU values above the cutoff for each antigen were analyzed.
b
Values showing statistically significant differences between skin test-positive and -negative animals are in bold.
c
P < 0.0001.
d
P < 0.05.