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. 2017 Feb 9;7:41772. doi: 10.1038/srep41772

Figure 4. Antiplasmodial activity of DEFB130.

Figure 4

(A) Morphological changes in the parasites after 3 h of culture in the presence of 50 μM peptide. Blood smears were stained with Giemsa stain. Scale bar = 10 μm. (B) Detection of DEFB130 in iRBCs by ELISA at different time points of incubation. Each bar represents the mean ± SEM for each group (n = 4) and the results are from individual experiment and are representative of two independent experiments. (C) Detection of DEFB130 in iRBCs after a 30-min treatment. Extracellular and late-ring stage parasites (upper panels), trophozoite (middle panels) and schizont (lower panels). Fixed blood smears were stained with specific antibodies and examined by confocal microscopy. Arrows indicate the parasites. Scale bar = 5 μm. Green indicates specific reaction with DEFB130 antibody and blue indicates Hoechst nuclear staining. (D) Parasitemia of C57/BL6 mice after treatment with DEFB130 peptide. Mice were infected with P. yoelii 17X-NL and on days 4, 5, and 6 p.i. were intravenously injected with DEFB130, sDEFB130, or PBS. Arrows indicate the time of injection. Parasitemia was monitored daily and on days 7 and 8 p.i. significant differences were observed. Each bar represents the mean ± SEM for each group (n = 5). *Indicates a significant difference between the groups as analyzed by one-way ANOVA.