Table 1. Selected up-regulated genes of macrophages phagocytizing iRBC as determined by DNA microarray analysis and qRT-PCR.
Gene | Genbank Accession number | Fold changeaa | ||
---|---|---|---|---|
Microarray | qRT-PCRb | qRT-PCRc | ||
SMTNL2 | NM_198501 | 4.613 | 7.20 ± 8.59 | 14.57 ± 11.72 |
DEFB130 | NM_001037804 | 4.569 | 4.49 ± 3.93 | 3.45 ± 1.75 |
RIT2 | NM_002930 | 4.543 | 5.74 ± 2.53 | 4.11 ± 6.60 |
SYTL4 | NM_080737 | 4.459 | 5.87 ± 3.77 | 6.20 ± 4.60 |
PRSS41 | NM_001135086 | 3.912 | 1.71 ± 0.85 | 3.44 ± 3.06 |
GRP | NM_002091 | 2.726 | 4.62 ± 4.95 | 3.38 ± 1.80 |
ADAM19 | NM_033274 | 2.209 | 1.54 ± 1.30 | 1.72 ± 0.73 |
aFold change indicates the mean expression level of the gene in macrophages co-cultured with iRBCs normalized to that in macrophages co-cultured with RBCs.
bResults of qRT-PCR are from the same samples used for the microarray. Results represent the mean fold change ± SEM of three independent experiments.
cResults of qRT-PCR are from pooled data of samples collected from three individual donors. Results represent the mean fold change ± SEM (n = 3).