Skip to main content
. 2017 Feb 9;7:42265. doi: 10.1038/srep42265

Figure 1. Sirt1 supports endothelial Cav1 expression via downregulation of miR-204.

Figure 1

In situ hybridization for miR-204 (A,C) and immunofluorescence for Cav1 (B,D) in thoracic aorta (A,B) and mesenteric resistance arteries (MRA) (C,D) of eSirt1−/− and Sirt1flx/flx mice systemically infused with miR-204 inhibitor oligonucleotide (miR-204 I) or scrambled control oligonucleotide (miR SC). Quantification of endothelial Cav1 is shown below immunofluorescence images. Red arrows point to miR-204. *p < 0.05 between eSirt1−/− + miR SC vs. Sirt1flx/flx + miR SC, Sirt1flx/flx + miR-204 I and eSirt1−/− + miR-204 I. #p < 0.05 between eSirt1−/− + miR-204 I vs. Sirt1flx/flx + miR SC, Sirt1flx/flx + miR-204 I. (E) Immunoblots for Sirt1 and Cav1 in HUVEC transfected with siRNA for Sirt1 or scrambled control siRNA (siRNA SC), and miR-204 I or miR SC. Data is representative of three independent experiments and densitometric quantification shown on right. *p < 0.05 between miR SC + siRNA Sirt1 vs. miR SC + siRNA SC, miR-204 I + siRNA SC and miR-204 I + siRNA Sirt1. #p < 0.05 between miR-204 I + siRNA Sirt1 vs. miR SC + siRNA SC, miR-204 I + siRNA SC.