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(A1–A3) Bax immunohistochemistry; (B1–B3) Bcl-2 immunohistochemistry; (C1–C3) CD-45 immunohistochemistry; (D1–D3): Caspase-3 immunohistochemistry; (E1–E3): TUNEL positivity; (A1–E1) diabetic control; (A2–E2) diabetes + ischemia-reperfusion; (A3–E3) diabetes + mangiferin 40 mg/kg/day + ischemia-reperfusion; n = 3 per group; 40X; scale bar 50 μm; (→): positive stain.
