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. 2017 Feb 9;7:42298. doi: 10.1038/srep42298

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Copyright © 2017, The Author(s)

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(A) Protein patterns in murine, porcine and bovine OFS (from top to bottom). Five biological replicates. Ma = marker; 1–5 = animal 1–5; C = cell lysate (epithelial cells after isolation from oviduct tissue); Me = growth medium. (B) Immunodetection of oviductin (OVGP1) in murine, porcine and bovine (from left to right) OFS after SDS-PAGE and Western Blot. 1–5 = OFS from donor animal 1–5. (C) Immunodetection of oviductin (OVGP1) in murine, porcine and bovine OFS after glycosidase digestion, SDS-PAGE and Western Blot. 1 = murine, 2 = porcine, 3 = bovine OFS, respectively; -S = digested with sialidase; -P = digested with PGNaseF.