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. 2017 Feb 9;7:42047. doi: 10.1038/srep42047

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(a) Scheme of the TALE-CIB1 fusion protein (~172 kD) containing mCherry as the fluorescent marker. (b) Scheme of the TET1-CD-CRY2-EGFP (TET1_-CRY2-EGFP) fusion protein (175 kD). (c) Scheme of the DNMT3A-CD-CRY2-EGFP (DNMT3A_-CRY2-EGFP) fusion protein (128 kD). (d–f) Representative co-expressing TET1_-CRY2-EGFP fusion protein (d, green) and TALE-CIB1-mcherry fusion protein (e, red) with merged image (f, orange and green) in a 2 day differentiated rat neuronal stem cell. We observed that TALE-CIB1 fusion proteins were predominantly expressed in the cytoplasm, while TET1_-CRY2-EGFP fusion proteins were expressed throughout the whole cell body and axon. White arrow: axon. EF1a: Elongation factor 1-alpha promoter; NLS: nuclear localization signal; EGFP: Enhanced Green Fluorescent Protein.